Applications of one-bead one-compound combinatorial libraries and chemical microarrays in signal transduction research

Acc Chem Res. 2003 Jun;36(6):370-7. doi: 10.1021/ar0201299.

Abstract

The "one-bead-one-compound" (OBOC) combinatorial library method synthesizes millions of random compounds such that each bead displays only one compound. Bead libraries are screened, and positive beads are isolated for structure analysis. Peptide substrates and inhibitors of protein kinases, and peptide ligands for cell surface receptors have been identified using this method. A novel encoding strategy for OBOC libraries has been developed to identify peptidomimetic and small-molecule ligands that specifically interact with cellular proteins. These ligands will be tested for their effects on cell signaling and used to construct chemical microarrays for further characterization of ligand-protein interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Combinatorial Chemistry Techniques*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology
  • Membrane Proteins / metabolism
  • Peptide Library
  • Protein Array Analysis / methods*
  • Protein Kinase Inhibitors
  • Protein Kinases / metabolism
  • Signal Transduction*
  • Substrate Specificity

Substances

  • Enzyme Inhibitors
  • Membrane Proteins
  • Peptide Library
  • Protein Kinase Inhibitors
  • Protein Kinases