Abstract
Cultivated CD4+ T-helper cells from two patients with cervical adenocarcinoma showed responses to a peptide EKTGILTVTYHSETQRTK derived from an E2 protein of human papillomavirus type 18 (HPV 18), but not to a corresponding HPV 16 peptide (HKSAIVTLTYDSEWQRDQ). Serum antibodies in the HPV 18 peptide were also demonstrated in these patients. The GILT motif resembles a common pattern present in many T-cell epitopes, and is located at the beginning of an 11-amino acid-long A-helix structure close to the carboxyterminal end of HPV 18 E2. We conclude that two epitopes (a T-helper cell epitope and a B-cell epitope) overlap in the HPV 18 E2.
MeSH terms
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Amino Acid Sequence
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Antibodies, Viral / immunology*
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B-Lymphocytes / immunology*
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DNA-Binding Proteins*
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Epitopes
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HLA-A Antigens / metabolism
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HLA-DR Antigens / metabolism
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Humans
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Lymphocyte Activation
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Molecular Sequence Data
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Oncogene Proteins, Viral / immunology*
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Peptides / immunology
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Receptors, Antigen, T-Cell / metabolism
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T-Lymphocytes / immunology*
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T-Lymphocytes, Helper-Inducer / immunology*
Substances
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Antibodies, Viral
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DNA-Binding Proteins
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E2 protein, Human papillomavirus type 16
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Epitopes
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HLA-A Antigens
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HLA-DR Antigens
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Oncogene Proteins, Viral
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Peptides
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Receptors, Antigen, T-Cell
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oncogene protein E2, Human papillomavirus type 18