Abstract
A carboxylated pullulan, for use as a structural material for a number of tissue engineering applications, was synthesized and conjugated with heparin. By immobilization of heparin to pullulan, endothelial cells (ECs) attached on the heparin-conjugated pullulan were more aggregated than when attached to other pullulan derivatives. Attachments were 50, 45, 49, and 90% for a polystyrene dish, pullulan acetate, carboxylated pullulan, and heparin-conjugated pullulan, respectively. Heparin-conjugated pullulan inhibited the proliferation of smooth muscle cells (SMCs) in vitro. Heparin-conjugated pullulan material can thus be used for the proliferation of vascular ECs and to inhibit the proliferation of SMCs.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Adhesion / physiology
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Cell Aggregation / physiology
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Division / physiology
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Cells, Cultured
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Cells, Immobilized / physiology
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Coated Materials, Biocompatible / chemical synthesis
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Endothelium, Vascular / physiology*
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Extracellular Matrix / chemistry*
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Extracellular Matrix / physiology
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Glucans / chemistry*
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Heparin / chemistry*
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Humans
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Macromolecular Substances
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Membranes, Artificial
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Muscle, Smooth, Vascular / physiology
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Rats
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Rats, Sprague-Dawley
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Tissue Engineering / instrumentation*
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Tissue Engineering / methods
Substances
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Coated Materials, Biocompatible
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Glucans
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Macromolecular Substances
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Membranes, Artificial
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pullulan
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Heparin