A new chromosomal breakpoint in Ph positive, bcr negative chronic myelogenous leukemia. Report of a case

M Negrini; A Tallarico; I Pazzi; A Castagnoli; A Cuneo; G L Castoldi

doi:10.1016/0165-4608(92)90363-d

A new chromosomal breakpoint in Ph positive, bcr negative chronic myelogenous leukemia. Report of a case

Cancer Genet Cytogenet. 1992 Jul 1;61(1):11-3. doi: 10.1016/0165-4608(92)90363-d.

Authors

M Negrini¹, A Tallarico, I Pazzi, A Castagnoli, A Cuneo, G L Castoldi

Affiliation

¹ Interdepartment Center for Cancer Research, University of Ferrara, Italy.

PMID: 1353407
DOI: 10.1016/0165-4608(92)90363-d

Abstract

We report a new case of Ph positive chronic myeloid leukemia (CML) without the classical rearrangement in Mbcr. By Southern blot analysis the molecular breakpoint was mapped 3 to 8 kb upstream of Mbcr. This region has not been shown to be rearranged in any other described case of CML. We did not detect any specific abnormal BCR-ABL transcript even with the use of the very sensitive RNA-PCR technique.

Publication types

Case Reports

MeSH terms

Base Sequence
Blood Cell Count
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 22*
Chromosomes, Human, Pair 9
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
Male
Middle Aged
Molecular Sequence Data
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Protein-Tyrosine Kinases*
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-bcr
Translocation, Genetic

Substances

Proto-Oncogene Proteins
Protein-Tyrosine Kinases
BCR protein, human
Proto-Oncogene Proteins c-bcr