Electrophysiological characterization of a TTX-sensitive sodium current in native Xenopus oocytes

Proc Biol Sci. 1992 Nov 23;250(1328):127-32. doi: 10.1098/rspb.1992.0140.

Abstract

We have studied a fast inward current expressed in oocytes from one Xenopus laevis. This current was characterized as a sodium current. It was activated by depolarizations to -50 mV or higher, peaked within 3-5 ms, and then decayed following a mono-exponential timecourse. When clamped at different holding potentials, the current displayed voltage-dependent inactivation with a V0.5 of -51 mV. The channel responsible for this Na+ entry was blocked by tetrodotoxin with a K0.5 of 8 nM, and was resistant to block by lidocaine at concentrations up to 100 microM. The pharmacological similarities between neuronal and oocyte sodium channels suggest that the two channels share a conserved structure.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electrophysiology / methods
  • Female
  • Kinetics
  • Lidocaine / pharmacology
  • Membrane Potentials / drug effects
  • Oocytes / drug effects
  • Oocytes / physiology*
  • Sodium / metabolism
  • Sodium Channels / drug effects
  • Sodium Channels / physiology*
  • Tetrodotoxin / pharmacology*
  • Time Factors
  • Xenopus laevis

Substances

  • Sodium Channels
  • Tetrodotoxin
  • Lidocaine
  • Sodium