Abstract
In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenine / metabolism
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Animals
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Base Sequence
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Binding Sites
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Catalysis
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Guanosine / metabolism
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Hydrogen Bonding
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Introns
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Methylation
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Molecular Sequence Data
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Mutagenesis
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Nucleic Acid Conformation
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Oligonucleotides / metabolism
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RNA / chemistry
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RNA / metabolism*
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RNA, Catalytic / chemistry
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RNA, Catalytic / genetics
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RNA, Catalytic / metabolism*
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Substrate Specificity
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Tetrahymena thermophila / genetics*
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Thermodynamics
Substances
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Oligonucleotides
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RNA, Catalytic
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Guanosine
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RNA
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Adenine