Abstract
Four sulfonic acid polymers [poly(4-styrenesulfonic acid)(PSS), poly(anetholesulfonic acid)(PAS), poly(vinylsulfonic acid)(PVS), poly(2-acrylamido-2-methyl-1-propanesulfonic acid)(PAMPS)] have been found to inhibit the cytopathicity of HIV-1 and HIV-2 in MT-4 cells at concentrations that are not toxic to the host cells. The sulfonic acid polymers also inhibited syncytium formation in co-cultures of MOLT-4 cells with HIV-1- or HIV-2-infected HUT-78 cells. They also inhibited binding of anti-gp120 mAb to HIV-1 gp120 and blocked adsorption of HIV-1 virions to MT-4 cells. PSS and PAS, but not PVS and PAMPS, interfered with the binding of OKT4A/Leu3a to the CD4 receptor. The anti-HIV activity of these polyanionic compounds can be ascribed to inhibition of the gp120-CD4 interaction. Sulfonic acid polymers represent a lead of anti-HIV compounds that warrant further evaluation of their therapeutic potential.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / metabolism
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Antiviral Agents / pharmacology*
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CD4 Antigens / immunology
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Cells, Cultured
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Flow Cytometry
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Fluorescent Antibody Technique
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Glycoproteins / immunology
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HIV / drug effects*
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HIV / metabolism
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HIV-1 / drug effects
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HIV-1 / metabolism
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HIV-2 / drug effects
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HIV-2 / metabolism
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Polymers / chemical synthesis
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Polymers / pharmacology*
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Polyvinyls / chemical synthesis
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Polyvinyls / pharmacology*
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RNA-Directed DNA Polymerase / metabolism
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Reverse Transcriptase Inhibitors
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Sulfonic Acids / chemical synthesis
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Sulfonic Acids / pharmacology*
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Virus Replication / drug effects
Substances
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Antiviral Agents
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CD4 Antigens
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Glycoproteins
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Polymers
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Polyvinyls
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Reverse Transcriptase Inhibitors
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Sulfonic Acids
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lyapolate
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poly(2-acrylamido-2-methyl-1-propanesulfonic acid)
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poly(anetholesulfonic acid)
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RNA-Directed DNA Polymerase
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styrenesulfonic acid polymer