Synthesis of linked carbohydrates and evaluation of their binding for 16S RNA by mass spectrometry

Bioorg Med Chem Lett. 2003 Nov 17;13(22):3915-8. doi: 10.1016/j.bmcl.2003.09.005.

Abstract

A library of linked molecules were synthesized from the common sugar moieties existing in the natural amino glycosides. These linked molecules were screened against bacterial 16S RNA for their binding affinity using a mass spectrometry-based technology. Some of these compounds exhibited low micromolar affinity and could serve as leads for further development as antibacterial agents.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Escherichia coli / genetics
  • Mass Spectrometry
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry*
  • RNA, Bacterial / chemistry
  • RNA, Ribosomal, 16S / chemistry*

Substances

  • Oligosaccharides
  • RNA, Bacterial
  • RNA, Ribosomal, 16S