[Detection of cytomegalovirus infection by polymerase chain reaction in hematopoietic stem cell transplantation recipients]

Zhonghua Xue Ye Xue Za Zhi. 2003 Aug;24(8):407-9.
[Article in Chinese]

Abstract

Objective: To evaluate the detection of cytomegalovirus (CMV) by polymerase chain reaction (PCR) for predicting the development of CMV disease.

Methods: One hundred and thirty one allo-HSCT patients performed in the past 2 years were analyzed retrospectively. PCR-CMV was used to monitor CMV viremia and vireuria once a week after transplantation.

Results: In the dynamic detection, CMV viremia was positive for at least one chance in 89 patients, vireuria did in 99 patients. Thirty-seven patients developed CMV disease with an accumulative incidence of 32.5%. The incidence of CMV disease was 15.6% in plasma CMV-PCR negative group, 31.3% in positive once group, and 47.3% in positive over twice group. There was significant difference among the three groups (P = 0.0126). The incidence of CMV disease was 24.8% in urine CMV-PCR negative group, 43.5% in positive once group, and 33.0% in positive over twice group, being no significant difference among them (P = 0.845). On analysis, viremia could predict the development of CMV disease: the PPV (positive predictive value) is 40.5%, NPV (negative predictive value) is 84.4%, sensitivity is 75.0%, and specificity is 69.2%.

Conclusions: Detected by CMV-PCR, MCV viremia may predict the development of CMV disease, but MCV vireuria cannot.

Publication types

  • English Abstract

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Cytomegalovirus / genetics
  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus Infections / diagnosis*
  • Cytomegalovirus Infections / etiology
  • DNA, Viral / blood
  • DNA, Viral / urine
  • Female
  • Hematopoietic Stem Cell Transplantation* / adverse effects
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods*
  • Retrospective Studies
  • Sensitivity and Specificity
  • Transplantation, Homologous / adverse effects

Substances

  • DNA, Viral