The application of AgNOR technique on glycol-methacrylate embedded human normal and pathological tissues

Pathologica. 1992 May-Jun;84(1091):317-24.

Abstract

The Authors have applied the AgNOR technique to a series of normal and pathological human tissues fixed in neutral buffered formalin and methacarnoy and embedded in glycol-methacrylate. The results show the compatibility of the AgNOR technique with this embedding medium as well as the possibility to obtain an adequate visualization of the specific silver precipitates (AgNORs) on a unique focal plane without appreciable nuclear overlap, making thus the valuation of the AgNORs less subjective.

MeSH terms

  • Digestive System / anatomy & histology
  • Fixatives
  • Humans
  • Methacrylates*
  • Neoplasms / pathology*
  • Nucleolus Organizer Region / ultrastructure*
  • Plastic Embedding*
  • Silver Staining*
  • Specimen Handling
  • Thyroid Gland / anatomy & histology

Substances

  • Fixatives
  • Methacrylates
  • hydroxyethyl methacrylate