Abstract
p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Nucleus / metabolism
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Cells, Cultured
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / metabolism
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Gene Expression Regulation*
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Genes, Tumor Suppressor
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Humans
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In Vitro Techniques
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Protein Binding
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Recombinant Proteins / metabolism
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Species Specificity
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Structure-Activity Relationship
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TATA Box*
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TATA-Box Binding Protein
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Transcription Factors / metabolism*
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Transcription, Genetic*
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Tumor Suppressor Protein p53 / metabolism*
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Recombinant Proteins
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TATA-Box Binding Protein
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Transcription Factors
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Tumor Suppressor Protein p53