Measurement of 18F-FDG concentrations in blood samples: comparison of direct calibration and standard solution methods

J Nucl Med Technol. 2003 Dec;31(4):206-9.

Abstract

Objective: The purpose of this study was to compare the accuracy and reliability of 2 well counter methods for measuring the activity concentration of (18)F-FDG in blood samples.

Methods: Three to 5 blood samples from 154 patient studies were weighed and measured in a well counter. The (18)F-FDG activity concentration was derived using, first, a direct calibration factor to convert measured well counter readings into activity concentration and, second, a comparison of measured counts with those of a specified standard solution.

Results: The ratio between the activity concentration results of the 2 methods was 0.996 +/- 0.033, indicating that the methods provided equal results.

Conclusion: Because the standard solution method is more prone to human error, less reproducible, and more labor intensive, preference should be given to the direct calibration method.

Publication types

  • Comparative Study
  • Evaluation Study
  • Validation Study

MeSH terms

  • Blood Chemical Analysis / methods*
  • Blood Chemical Analysis / standards
  • Calibration
  • Fluorodeoxyglucose F18 / blood*
  • Fluorodeoxyglucose F18 / pharmacokinetics*
  • Humans
  • Radioisotope Dilution Technique / instrumentation*
  • Radiometry / instrumentation*
  • Radiometry / methods*
  • Radiopharmaceuticals / blood
  • Radiopharmaceuticals / pharmacokinetics
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Radiopharmaceuticals
  • Fluorodeoxyglucose F18