Abstract
To study the GH3 gene family of Arabidopsis, we investigated a flanking sequence database of Arabidopsis activation-tagged lines. We found a dwarf mutant, named yadokari 1-D (ydk1-D), that had a T-DNA insertion proximal to a GH3 gene. ydk1-D is dominant and has a short hypocotyl not only in light but also in darkness. Moreover, ydk1-D has a short primary root, a reduced lateral root number, and reduced apical dominance. A GH3 gene, named YDK1, was upregulated in ydk1-D, and YDK1 transgenic plants showed the ydk1-D phenotype. YDK1 gene expression was induced by exogenously applied auxin and regulated by auxin-response factor (ARF)7. In addition, YDK1 gene expression was downregulated by blue and far-red (FR) lights. Strong promoter activity of YDK1 was observed in roots and flowers. These results suggest that YDK1 may function as a negative component in auxin signaling by regulating auxin activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / drug effects
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Developmental / radiation effects
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Gene Expression Regulation, Plant / drug effects
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Gene Expression Regulation, Plant / radiation effects
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Hypocotyl / drug effects
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Hypocotyl / genetics
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Hypocotyl / growth & development*
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Indoleacetic Acids / pharmacology*
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Light
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Multigene Family
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Phenotype
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Plant Growth Regulators / genetics
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Plant Growth Regulators / metabolism
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Plant Growth Regulators / pharmacology
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Plant Roots / drug effects
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Plant Roots / genetics
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Plant Roots / growth & development*
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Plants, Genetically Modified
Substances
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Arabidopsis Proteins
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DFL1 protein, Arabidopsis
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DFL2 protein, Arabidopsis
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Indoleacetic Acids
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Plant Growth Regulators
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YDK1 protein, Arabidopsis