Abstract
MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrosome / physiology
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Amino Acid Sequence
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Antibodies, Monoclonal
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Antigens, CD / chemistry*
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Antigens, Surface / chemistry*
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Antigens, Surface / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Humans
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Male
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Membrane Cofactor Protein
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Membrane Glycoproteins / chemistry*
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Molecular Sequence Data
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Precipitin Tests
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Semen
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Sequence Homology, Amino Acid
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Spermatozoa / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Surface
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CD46 protein, human
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Membrane Cofactor Protein
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Membrane Glycoproteins