Characterization of high-salt and high-fat diets on cardiac and vascular function in mice

Cardiovasc Toxicol. 2004;4(1):37-46. doi: 10.1385/ct:4:1:37.

Abstract

This study compared two established dietary formulations, high salt and high fat-high carbohydrate, separately or in combination on the induction cardiovascular dysfunction. One-month-old C57BL/6J mice were fed one of the following diets for 3 mo: (1) control diet consisting of a high fat-high sim-ple carbohydrate (HFHSC); (2) 8% NaCl diet (HS); or (3) HFHSC diet supplemented with 1% NaCl (HFHS). After 3 mo, the HFHSC mice demonstrated significantly increased end-diastolic volume and end-systolic volume, specifically increases of 35% and 78%, respectively (p < 0.01) and a reduction of ventricular stiffness by 27% (p = 0.015). The HS mice exhibited arterial hyper-tension with an increase of 33% in maximum end-systolic pressure (p =.024) and a decrease of 44% in arterial elastance (p = 0.020), corroborated by an increase in the heart weight to body weight ratios (p = 0.002) and vascular types I and III collagen (p = 0.03 and p = 0.0008, respectively). The HFHS group revealed a striking response of 230% to the alpha1-adrenergic challenge (p = 0.00034). These data suggest that the HFHSC diet causes dilated cardio-myopathy, whereas the HS diet produces arterial hypertension and the HFHS diet causes a vascular dysfunctional state that was highly responsive to alpha-adrenergic stimulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arteries / drug effects
  • Collagen / biosynthesis
  • Collagen / genetics
  • Diet
  • Dietary Carbohydrates / pharmacology
  • Dietary Fats / pharmacology*
  • Heart / drug effects*
  • Heart Diseases / etiology
  • Heart Diseases / physiopathology
  • Hemodynamics / drug effects*
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium Chloride, Dietary / pharmacology*
  • Ventricular Function, Left / drug effects

Substances

  • Dietary Carbohydrates
  • Dietary Fats
  • RNA, Messenger
  • Sodium Chloride, Dietary
  • Collagen