An electronic pressure-meter nociception paw test for mice

Braz J Med Biol Res. 2004 Mar;37(3):401-7. doi: 10.1590/s0100-879x2004000300018. Epub 2004 Mar 3.

Abstract

The aim of the present investigation was to describe and validate an electronic mechanical test for quantification of the intensity of inflammatory nociception in mice. The electronic pressure-meter test consists of inducing the animal hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared to the classical von Frey filaments test in which pressure intensity is automatically recorded after the nociceptive hindpaw flexion reflex. The electronic pressure-meter and the von Frey filaments were used to detect time versus treatment interactions of carrageenin-induced hypernociception. In two separate experiments, the electronic pressure-meter was more sensitive than the von Frey filaments for the detection of the increase in nociception (hypernociception) induced by small doses of carrageenin (30 microg). The electronic pressure-meter detected the antinociceptive effect of non-steroidal drugs in a dose-dependent manner. Indomethacin administered intraperitoneally (1.8-15 mg/kg) or intraplantarly (30-300 microg/paw) prevented the hypersensitive effect of carrageenin (100 microg/paw). The electronic pressure-meter also detected the hypernociceptive effect of prostaglandin E2 (PGE2; 10-100 ng) in a dose-dependent manner. The hypernociceptive effect of PGE2 (100 ng) was blocked by dipyrone (160 and 320 microg/paw) but not by intraplantar administration of indomethacin (300 microg/paw). The present results validate the use of the electronic pressure-meter as more sensitive than the von Frey filaments in mice. Furthermore, it is an objective and quantitative nociceptive test for the evaluation of the peripheral antinociceptive effect of anti-inflammatory analgesic drugs, which inhibit prostaglandin synthesis (indomethacin) or directly block the ongoing hypernociception (dipyrone).

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Analysis of Variance
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Carrageenan / pharmacology
  • Dinoprostone / pharmacology
  • Dipyrone / pharmacology
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Indomethacin / pharmacology
  • Mice
  • Pain Measurement / instrumentation
  • Pain Measurement / methods*
  • Pressure
  • Reaction Time
  • Sensitivity and Specificity

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Dipyrone
  • Carrageenan
  • Dinoprostone
  • Indomethacin