Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases

Paul F van Swieten; Rene Maehr; Adrianus M C H van den Nieuwendijk; Benedikt M Kessler; Michael Reich; Chung-Sing Wong; Hubert Kalbacher; Michiel A Leeuwenburgh; Christoph Driessen; Gijsbert A van der Marel; Hidde L Ploegh; Herman S Overkleeft

doi:10.1016/j.bmcl.2004.04.046

Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases

Bioorg Med Chem Lett. 2004 Jun 21;14(12):3131-4. doi: 10.1016/j.bmcl.2004.04.046.

Authors

Paul F van Swieten¹, Rene Maehr, Adrianus M C H van den Nieuwendijk, Benedikt M Kessler, Michael Reich, Chung-Sing Wong, Hubert Kalbacher, Michiel A Leeuwenburgh, Christoph Driessen, Gijsbert A van der Marel, Hidde L Ploegh, Herman S Overkleeft

Affiliation

¹ Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.

PMID: 15149659
DOI: 10.1016/j.bmcl.2004.04.046

Abstract

Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Cysteine Endopeptidases / analysis
Cysteine Endopeptidases / metabolism*
Isotope Labeling / methods*
Isotopes / chemical synthesis
Isotopes / metabolism
Proteomics / methods

Substances

Isotopes
Cysteine Endopeptidases

Grants and funding

GM 62502/GM/NIGMS NIH HHS/United States