Interleukin-10 and interleukin-6 gene polymorphisms as risk factors for Alzheimer's disease

Neurobiol Aging. 2004 Sep;25(8):1009-15. doi: 10.1016/j.neurobiolaging.2003.10.009.

Abstract

In the pathogenesis of Alzheimer disease (AD), it has been proposed that the anti-inflammatory interleukins such as IL-10 regulate beta-amyloid-induced microglial inflammatory responses inhibiting the proinflammatory cytokine IL-6. Since the promoters of the IL-10 and IL-6 genes show single nucleotide polymorphisms (SNPs) (IL-10: -1082 G --> A; IL-6: -174 G --> C), we investigated these SNPs and cytokine production by peripheral blood mononuclear cells in 65 AD patients and 65 controls (HC). In AD there was a significant increase of the -1082A IL-10 allele (P=0.009) and a decrease of -1082GG genotype (P=0.019). The frequency of the GG IL-6 genotype in AD was lower and the C allele significantly higher (P <0.005). The co-occurrence of IL-10 A and IL-6 C alleles significantly raised the odds ratio (OR 11.2, confidence interval: CI 1.3-97.3; P <0.05) independently of apolipoprotein E4 (adjusted OR 10.3, CI 1-108; P <0.05). Only amyloid-stimulated IL-10 production differed between the groups (P=0.023). These results raise questions regarding the inflammatory theory in AD, pointing to a pivotal role of IL-10 and IL-6 and a selective alteration in this network.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / genetics*
  • Alzheimer Disease / immunology*
  • Alzheimer Disease / physiopathology
  • Amyloid beta-Peptides / metabolism
  • Apolipoproteins E / metabolism
  • Brain / immunology
  • Brain / metabolism
  • Brain / physiopathology
  • DNA Mutational Analysis
  • Encephalitis / genetics
  • Encephalitis / immunology
  • Encephalitis / physiopathology
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease / genetics*
  • Genetic Testing
  • Genotype
  • Humans
  • Interleukin-10 / genetics*
  • Interleukin-10 / immunology
  • Interleukin-6 / genetics*
  • Interleukin-6 / immunology
  • Italien
  • Male
  • Polymorphism, Genetic / genetics*
  • Polymorphism, Single Nucleotide / genetics
  • Promoter Regions, Genetic / genetics
  • Risk Factors

Substances

  • Amyloid beta-Peptides
  • Apolipoproteins E
  • Interleukin-6
  • Interleukin-10