Abstract
We report the cloning of human GluR4c, an alternative splicing isoform of GluR4. Similar to rodent and chick GluR4c mRNA, human GluR4c had a 113-bp insert containing a stop codon, resulting in a short C terminus. The expression of human GluR4c was widespread in the brain, and was upregulated with development in the cerebellum and cerebral cortex where the level of it was about 30% of total GluR4 mRNA in adult stage. The GluR4 subunit may play a pivotal role in regulating channel properties as well as trafficking of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors in the adult human brain.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Alternative Splicing*
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Animals
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Brain / anatomy & histology
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Brain / growth & development
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Brain / metabolism*
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Chickens
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Female
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Fetus
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Gene Expression Regulation, Developmental*
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Humans
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In Situ Hybridization / methods
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Infant
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Infant, Newborn
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Male
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Middle Aged
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Postmortem Changes
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Protein Isoforms / genetics
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Protein Isoforms / metabolism*
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RNA, Messenger / biosynthesis
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Rats
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Receptors, AMPA / genetics
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Receptors, AMPA / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sequence Analysis, DNA / methods
Substances
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Protein Isoforms
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RNA, Messenger
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Receptors, AMPA
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glutamate receptor ionotropic, AMPA 4