Objective: To study the abnormity and clinical value of tyrosine kinase signaling pathway in lung adenocarcinoma.
Methods: Total RNA was extracted form 10 lung adenocarcinoma samples and matched normal tissues. cDNA was labeled fluorescence during reverse transcription and hybridized on 20 slides of microarray with 13 824 genes. After washed and scanned, the cy3:cy5 ratios were obtained by computer analysis. Each experiment was repeated two times and the mean value was chosen. Cluster analysis and discriminant analysis were used to mine the pool data. The results were combined with clinical prognostic factors for further analysis.
Results: According to the abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma, sample cluster divided the 10 samples into 3 groups and gene cluster divided all genes into 5 groups. Different prognosis samples had different characteristic genes. The patients with the best prognosis overexpressed some genes related to immunity and inhibition proliferation, and those with the worst prognosis overexpressed some genes related to proliferation while the genes related to immunity and inhibition proliferation were expressed at a low level.
Conclusion: There are 3 type abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma that are related to different biological behavior and different prognosis.