Abstract
We have investigated cyclin A expression in a primary culture of normal rat hepatocytes and during rat liver regeneration after partial hepatectomy. In both cases, cyclin A mRNA and protein accumulate as the cells enter S phase. To investigate the potential implication of cyclin A accumulation at S phase, we microinjected anti-sense DNA constructs for cyclin A, resulting in effective inhibition of S phase entry. These effects were specific for cyclin A since anti-sense cyclin B construct had no similar effects. These results therefore, obtained in normal epithelial cells, indicate that cyclin A is involved in S phase and thus should not be only considered as a mitotic cyclin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoradiography
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Blotting, Northern
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Cells, Cultured
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Culture Media, Serum-Free
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Cyclins / genetics
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Cyclins / physiology*
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DNA Replication / drug effects
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DNA, Antisense / pharmacology
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Dexamethasone / pharmacology
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Epithelial Cells
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Epithelium / physiology
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Hepatectomy
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Immunohistochemistry
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Insulin / pharmacology
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Liver / cytology
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Liver / drug effects
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Liver / physiology*
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Male
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
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Rats, Inbred Strains
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S Phase* / drug effects
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Thymidine / metabolism
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Tritium
Substances
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Culture Media, Serum-Free
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Cyclins
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DNA, Antisense
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Insulin
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RNA, Messenger
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Tritium
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Dexamethasone
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Thymidine