Abstract
The flavoenzyme uridine 5'-diphosphate (UDP)-galactopyranose mutase (UGM) plays a key role in the cell wall biosynthesis of many pathogens, including Mycobacterium tuberculosis. Using a synthetic fluorescent ligand, we screened 16 000 compounds in a fluorescence polarization assay. Effective inhibitors of UGM were identified.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chromatography, High Pressure Liquid
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology*
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Fluorescence Polarization / methods
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Inhibitory Concentration 50
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Intramolecular Transferases / antagonists & inhibitors*
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Intramolecular Transferases / metabolism
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Kinetics
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Mycobacterium tuberculosis / enzymology
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Substrate Specificity
Substances
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Enzyme Inhibitors
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Intramolecular Transferases
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UDP-galactopyranose mutase