Background: To investigate preparation of human-derived anti-HBs IgG antibody.
Methods: mRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test.
Results: A whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant.
Conclusion: It can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.