Abstract
Vascular smooth muscle cells proliferate and transform to foam cells in the process of atherosclerosis. In the present study, we demonstrated that platelet-derived growth factor (PDGF)-BB induced expression of proto-oncogene c-fms in vascular smooth muscle cells, which normally do not express c-fms, isolated from either human umbilical artery or rabbit aorta. No effect of the protein kinase C activator, phorbol ester, was demonstrated on mRNA expression of c-fms. In contrast, the scavenger receptor activity was induced by both PDGF-BB and phorbol ester. These results indicate that two characteristic genes of monocyte-macrophages were induced by PDGF-BB via the different pathways, and suggest that PDGF-BB plays an important role in initiating phenotypic conversion of smooth muscle cells to macrophage-like cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arteriosclerosis / metabolism*
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Base Sequence
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Cells, Cultured
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DNA
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Humans
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Immunoblotting
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Kinetics
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Male
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Membrane Proteins*
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Microscopy, Fluorescence
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Molecular Sequence Data
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Muscle, Smooth, Vascular / cytology
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Muscle, Smooth, Vascular / metabolism*
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Platelet-Derived Growth Factor / physiology*
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Proto-Oncogene Mas
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RNA, Messenger / metabolism
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Rabbits
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Receptor, Macrophage Colony-Stimulating Factor / genetics*
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Receptor, Macrophage Colony-Stimulating Factor / metabolism
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Receptors, Immunologic / genetics*
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Receptors, Lipoprotein*
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Receptors, Scavenger
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Scavenger Receptors, Class B
Substances
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MAS1 protein, human
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Membrane Proteins
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Platelet-Derived Growth Factor
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Proto-Oncogene Mas
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Lipoprotein
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Receptors, Scavenger
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Scarb1 protein, mouse
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Scavenger Receptors, Class B
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DNA
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Receptor, Macrophage Colony-Stimulating Factor