Induction of anti-leukemic cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous myeloblasts

Leuk Res. 2004 Dec;28(12):1303-12. doi: 10.1016/j.leukres.2004.03.018.

Abstract

Presentation of AML antigens by dendritic cells (DC) could potentially induce a T cell-mediated anti-leukemic immune response. In the present study, we generated DC from adherent (AD-DC) and non-adherent (NAD-DC) myeloblasts obtained from bone marrows of AML patients. Both cell populations displayed morphological, phenotypic and functional properties of DC. The functions of NAD-DC were compared to AD-DC that had been fused with autologous AML blasts (FC/AML). The FC/AML induced greater T cell proliferation and CTL activity against autologous AML blasts (9/10 cases) as compared to NAD-DC. FC/AML may thus represent a promising strategy for DC-based immunotherapy of patients with AML.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Bone Marrow Cells
  • Cell Adhesion
  • Cell Culture Techniques
  • Cell Fusion
  • Cytotoxicity, Immunologic
  • Dendritic Cells / pathology*
  • Female
  • Granulocyte Precursor Cells / pathology*
  • Humans
  • Immunotherapy / methods
  • Leukemia / immunology*
  • Leukemia / pathology
  • Lymphocyte Activation*
  • Male
  • Middle Aged
  • T-Lymphocytes, Cytotoxic / immunology*