Abstract
The Simian Virus 40 (SV40) small-t antigen (ST) plays an important role in driving cell proliferation, enhancing transformation by the large-T (LT) antigen. Potential targets of ST are the cyclin kinase inhibitor p27 and the cyclin A gene itself. Transactivation of the cyclin A promoter by ST depends on the interaction of ST with protein phosphatase 2A (PP2A) and occurs through a cell cycle-regulated E2F site near the transcription start site of the promoter. A third SV40 early protein, 17KT, also transactivates the cyclin A promoter but, in this case, transactivation depends on the dnaJ domain of the protein.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Viral, Tumor / physiology*
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Cell Cycle / physiology*
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Cell Cycle Proteins / metabolism*
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Cell Line
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Chlorocebus aethiops
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Cyclin A / genetics*
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DNA-Binding Proteins / metabolism*
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E2F Transcription Factors
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Humans
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Phosphoprotein Phosphatases / metabolism*
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Promoter Regions, Genetic
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Protein Phosphatase 2
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Simian virus 40 / physiology*
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Transcription Factors / metabolism*
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Transcriptional Activation
Substances
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Antigens, Viral, Tumor
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Cell Cycle Proteins
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Cyclin A
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DNA-Binding Proteins
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E2F Transcription Factors
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Transcription Factors
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Phosphoprotein Phosphatases
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Protein Phosphatase 2