Direct enantiomeric resolutions of chiral triazole pesticides by high-performance liquid chromatography

J Biochem Biophys Methods. 2005 Mar 31;62(3):219-30. doi: 10.1016/j.jbbm.2004.11.007. Epub 2005 Jan 18.

Abstract

Cellulose-tris (3,5-dimethylphenylcarbamate; CDMPC) was synthesized and coated on aminopropylsilica to prepare chiral stationary phase (CSP). Normal-phase high-performance liquid chromatography (HPLC) methods for the resolutions of five chiral triazole pesticides, diniconazole, tebuconazole, hexaconazole, triadimefon and flutriafol, on the CSP were developed. Several operating parameters such as mobile phase composition, modifier and column temperature were studied for the optimization of the resolutions. Better separations were achieved using 2% iso-butanol for diniconazole, 2% ethanol for tebuconazole, 2% iso-propanol for hexaconazole, 1% n-butanol for triadimefon and 2% n-propanol for flutriafol as modifiers in n-hexane at 0 degrees C with the resolution factors (Rs) of 1.62, 1.66, 2.46, 1.68 and 1.98, respectively. Low temperature was better for the resolutions. Validation of the methods included linearity and precision.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Ether / chemistry
  • Molecular Structure
  • Pesticides / chemistry*
  • Pesticides / isolation & purification*
  • Petroleum
  • Solvents / chemistry
  • Stereoisomerism
  • Temperature
  • Triazoles / chemistry*
  • Triazoles / isolation & purification*

Substances

  • Pesticides
  • Petroleum
  • Solvents
  • Triazoles
  • Ether