Abstract
The driving forces of the HIV-1 epidemic in Guangxi, China were assessed by investigating virologic and epidemiologic data from a cohort of longitudinally followed injection drug users (IDUs) in Binyang and Pingxiang, major urban areas along 2 separate drug routes in the province. Sera and interview data were obtained in September and October of 2000. Sequence analysis of HIV-1 was performed on the gag-pol region (HXB2 nt 1850-3005) and C2 to V4 env (HXB2 nt 6704-7626). Sequence data demonstrated that 88% of the infections in Pingxiang were CRF01_AE, whereas 96% in Bin-yang were CRF08_BC. Three recently infected subjects in Pingxiang were infected with CRF08_BC, and 1 chronically infected subject had evidence of a recombinant virus. Intersubject distances were statistically greater for CRF01_AE-infected subjects than CRF08_BC-infected subjects for all regions except V4. The epidemic in Binyang is similar to previously described IDU-based epidemics, with a strong founder effect with little variation in V3. The epidemic in Pingxiang may have had multiple introductions of the CRF01_AE epidemic into the city and greater spread through sexual transmission, resulting in greater variation in V3 than typically seen in purely parenterally based epidemics.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Amino Acid Sequence
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China / epidemiology
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DNA, Complementary
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DNA, Viral / chemistry
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DNA, Viral / isolation & purification
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Female
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Fusion Proteins, gag-pol / genetics
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Gene Products, env / chemistry
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Genes, env
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Genes, gag
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Genes, pol
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HIV Infections / epidemiology*
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HIV Infections / virology*
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HIV Protease / chemistry
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HIV Protease / genetics
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HIV Reverse Transcriptase / chemistry
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HIV Reverse Transcriptase / genetics
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HIV-1 / classification*
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HIV-1 / genetics
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HIV-1 / isolation & purification*
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Humans
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Male
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Middle Aged
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Molecular Epidemiology
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Molecular Sequence Data
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Phylogeny
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Sequence Alignment
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Sequence Analysis, DNA
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Substance Abuse, Intravenous / complications
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Urban Population
Substances
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DNA, Complementary
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DNA, Viral
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Fusion Proteins, gag-pol
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Gene Products, env
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HIV Reverse Transcriptase
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HIV Protease
Associated data
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GENBANK/AY635691
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GENBANK/AY635692
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