Wnt/beta-catenin signaling acts upstream of N-myc, BMP4, and FGF signaling to regulate proximal-distal patterning in the lung

Dev Biol. 2005 Jul 1;283(1):226-39. doi: 10.1016/j.ydbio.2005.04.014.

Abstract

Branching morphogenesis in the lung serves as a model for the complex patterning that is reiterated in multiple organs throughout development. Beta-catenin and Wnt signaling mediate critical functions in cell fate specification and differentiation, but specific functions during branching morphogenesis have remained unclear. Here, we show that Wnt/beta-catenin signaling regulates proximal-distal differentiation of airway epithelium. Inhibition of Wnt/beta-catenin signaling, either by expression of Dkk1 or by tissue-specific deletion of beta-catenin, results in disruption of distal airway development and expansion of proximal airways. Wnt/beta-catenin functions upstream of BMP4, FGF signaling, and N-myc. Moreover, we show that beta-catenin and LEF/TCF activate the promoters of BMP4 and N-myc. Thus, Wnt/beta-catenin signaling is a critical upstream regulator of proximal-distal patterning in the lung, in part, through regulation of N-myc, BMP4, and FGF signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning / physiology*
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins / physiology*
  • Cell Differentiation
  • Cytoskeletal Proteins / physiology*
  • Fibroblast Growth Factors / physiology*
  • Gene Expression Regulation*
  • Intercellular Signaling Peptides and Proteins / physiology*
  • Lung / cytology
  • Lung / embryology*
  • Mice
  • Mice, Inbred C57BL
  • Morphogenesis
  • Protein-Tyrosine Kinases / physiology
  • Proto-Oncogene Proteins c-myc / physiology*
  • Signal Transduction
  • Trans-Activators / physiology*
  • Wnt Proteins
  • beta Catenin

Substances

  • Bmp4 protein, mouse
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins
  • CTNNB1 protein, mouse
  • Cytoskeletal Proteins
  • Intercellular Signaling Peptides and Proteins
  • Proto-Oncogene Proteins c-myc
  • Trans-Activators
  • Wnt Proteins
  • beta Catenin
  • Fibroblast Growth Factors
  • Protein-Tyrosine Kinases