[SNP genotyping by multiplex amplification and microarrays assay and forensic application]

Fa Yi Xue Za Zhi. 2005 May;21(2):90-5.
[Article in Chinese]

Abstract

Objective: Research on the application feasibility of SNP genotyping for forensic identification by microarrays.

Methods: Oligonucleotide microarrays which could detect 31 different SNPs were used. Population studies on 31 SNP loci was carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.

Results: No deviations from Hardy-Weinberg equilibrium could be found at the 31 SNP loci 4 loci were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal informative SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in duos and trios were 0.9609 and 0.9970 respectively.

Conclusion: The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asian People / genetics
  • DNA Fingerprinting / methods*
  • DNA Primers
  • Forensic Medicine / methods
  • Genotype
  • Humans
  • Oligonucleotide Array Sequence Analysis*
  • Paternity
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide*
  • Tandem Repeat Sequences

Substances

  • DNA Primers