Abstract
One unresolved issue in gut immunity is how mucosal T lymphocytes are activated and which antigen-presenting cell (APC) is critical for the regulation of this process. We have identified a unique population of APCs that is exclusively localized in the lamina propria. These APCs constitutively expressed the costimulatory molecule CD70 and had antigen-presenting functions. After oral infection of mice with Listeria monocytogenes, proliferation and differentiation of antigen-specific T cells occurred in the gut mucosa in situ and blockade of CD70 costimulation abrogated the mucosal T cell proliferation and effector functions. Thus, a potent CD70-dependent stimulation via specialized tissue-specific APCs is required for the proliferation and differentiation of gut mucosal T cells after oral infection.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen Presentation / immunology
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Antigens, CD / immunology*
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CD27 Ligand
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Cell Differentiation / immunology
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Flow Cytometry
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Immunohistochemistry
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Intestinal Mucosa / cytology
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Intestinal Mucosa / immunology*
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Intestinal Mucosa / microbiology
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Listeria monocytogenes / immunology*
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Listeriosis / immunology*
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Listeriosis / pathology
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Lymphocyte Activation / immunology
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Membrane Proteins / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Phagocytosis / immunology
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Specific Pathogen-Free Organisms
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology*
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / immunology
Substances
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Antigens, CD
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CD27 Ligand
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Cd70 protein, mouse
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Membrane Proteins
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Tumor Necrosis Factor Receptor Superfamily, Member 7