Histopathologic alterations following local delivery of dexamethasone to inhibit restenosis in murine arteries

Cardiovasc Res. 2005 Dec 1;68(3):415-24. doi: 10.1016/j.cardiores.2005.06.015. Epub 2005 Jul 15.

Abstract

Objective: Dexamethasone-eluting stents are currently under evaluation to prevent post-angioplasty restenosis. The efficacy and safety of dexamethasone as an anti-restenotic agent is still unclear. We assess the effect of perivascular delivery of dexamethasone on vascular pathology in a mouse model of restenosis.

Methods and results: In this study we investigate the ability of both systemic and local dexamethasone treatment to inhibit neointima formation after cuff placement around C57BL/6 mouse femoral artery. As in the clinical situation, systemic dexamethasone treatment shows adverse side effects in animals, including weight loss. In contrast, local delivery of dexamethasone using a drug-eluting polymer cuff inhibits neointima formation and has no systemic adverse effects. Pathobiological examination of the experimental arteries, however, reveals a dose-dependent medial atrophy, a reduction in vascular smooth muscle cells and collagen content, an increase in apoptotic cell count and disruption of the internal elastic lamina.

Conclusions: Our results demonstrate that although local dexamethasone delivery is effective as an inhibitor for neointima formation, it is dose-dependently associated with adverse vascular morphological changes pointing to a loss of vascular integrity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angioplasty, Balloon, Coronary
  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / therapeutic use
  • Apoptosis / drug effects
  • Cell Proliferation / drug effects
  • Collagen / analysis
  • Coronary Disease / therapy
  • Coronary Restenosis / prevention & control*
  • Dexamethasone / administration & dosage*
  • Dexamethasone / therapeutic use
  • Femoral Artery / pathology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Muscle, Smooth, Vascular / pathology
  • Stents*
  • Tunica Intima / pathology*

Substances

  • Anti-Inflammatory Agents
  • Dexamethasone
  • Collagen