A meiosis-specific cyclin regulated by splicing is required for proper progression through meiosis

Mol Cell Biol. 2005 Aug;25(15):6330-7. doi: 10.1128/MCB.25.15.6330-6337.2005.

Abstract

The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, a reductional pattern of chromosome segregation at the first division, and a second division with no intervening DNA synthesis. Cyclin-dependent kinases are essential for progression through the meiotic cell cycle, as for the mitotic cycle. Here we show that a fission yeast cyclin, Rem1, is present only during meiosis. Cells lacking Rem1 have impaired meiotic recombination, and Rem1 is required for premeiotic DNA synthesis when Cig2 is not present. rem1 expression is regulated at the level of both transcription and splicing, with Mei4 as a positive and Cig2 a negative factor of rem1 splicing. This regulation ensures the timely appearance of the different cyclins during meiosis, which is required for the proper progression through the meiotic cell cycle. We propose that the meiosis-specific B-type cyclin Rem1 has a central role in bringing about progression through meiosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / metabolism
  • Cyclin B / genetics
  • Cyclin B / metabolism*
  • Cyclins / genetics
  • Cyclins / metabolism
  • Gene Expression Regulation, Fungal / physiology
  • Meiosis / genetics
  • Meiosis / physiology*
  • RNA Splicing / genetics
  • RNA Splicing / physiology*
  • RNA, Messenger / metabolism
  • Recombination, Genetic
  • Schizosaccharomyces / physiology
  • Schizosaccharomyces pombe Proteins / metabolism

Substances

  • Cell Cycle Proteins
  • Cig2 protein, S pombe
  • Cyclin B
  • Cyclins
  • RNA, Messenger
  • Rem1 protein, S pombe
  • Schizosaccharomyces pombe Proteins