Comparison of GB virus C, HIV, and HCV infection markers in hemophiliacs exposed to non-inactivated or inactivated factor concentrates

J Clin Virol. 2005 Sep;34(1):42-7. doi: 10.1016/j.jcv.2005.01.008.

Abstract

Background: Until the mandatory introduction of viral inactivation techniques of blood plasma products in the early 1980s many recipients of these products were infected with various viral pathogens.

Objectives: To determine the rate of transmission of GB virus C/hepatitis G virus (GBV-C/HGV) HCV, and HIV through non-virus-inactivated clotting factor concentrates in hemophiliacs, as well as the relation between amount of administered clotting factor and risk for GBV-C/HGV infection.

Study design: In this cross-sectional study, we determined retrospectively the rates of infection markers for GBV-C/HGV, HCV, and HIV in a German cohort of hemophiliacs treated with documented amounts of non-virus-inactivated clotting factor concentrates (group A) and in a second group of hemophiliacs who were treated exclusively with virus-inactivated clotting factor (group B). The presence of anti-virus antibodies was determined by ELISA. Viral RNA was detected by RT-PCR. Markers for viral infections were compared to amounts of administered non-virus-inactivated clotting factor.

Results: Among hemophiliacs treated with documented amounts of non-virus-inactivated clotting factor the prevalence for GBV-C/HGV, HCV, and HIV was 40.3%, 98.6%, and 56.3%, respectively. In contrast to HIV, the rate of GBV-C/HGV infections did not increase with increasing amounts of consumed non-inactivated clotting factor. Even in the subgroup of heavily treated hemophiliacs the rate of GBV-C/HGV infection markers did not exceed 45%.

Conclusions: The amount of non-virus-inactivated clotting factor is not predictive for the risk of GBV-C/HGV infection in hemophiliacs. Despite repeated parenteral exposure more than 55% of hemophiliacs were not infected with GBV-C/HGV. Our findings indicate a high frequency of host factors preventing parenteral transmission of GBV-C/HGV.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / blood
  • Base Sequence
  • Cross-Sectional Studies
  • DNA Primers
  • Flaviviridae Infections / transmission*
  • GB virus C*
  • HIV Infections / transmission*
  • HIV*
  • Hemophilia A / blood
  • Hemophilia A / virology*
  • Hepacivirus*
  • Hepatitis C / transmission*
  • Humans
  • Polymerase Chain Reaction
  • RNA, Viral / blood*
  • RNA, Viral / isolation & purification
  • Retrospective Studies
  • Viral Load*

Substances

  • Antibodies, Viral
  • DNA Primers
  • RNA, Viral