Abstract
The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Amino Acid Sequence
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Animals
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Antineoplastic Agents / administration & dosage
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Cell Line, Tumor
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Depsipeptides / administration & dosage*
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Histone Deacetylase Inhibitors*
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Histones / chemistry
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Histones / metabolism*
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Hydroxamic Acids / administration & dosage*
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Lymphoma, Non-Hodgkin / drug therapy*
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Lymphoma, Non-Hodgkin / metabolism*
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Mice
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Molecular Sequence Data
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Peptide Mapping / methods*
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Protein Isoforms / chemistry
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Protein Isoforms / metabolism
Substances
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Antineoplastic Agents
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Depsipeptides
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Histone Deacetylase Inhibitors
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Histones
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Hydroxamic Acids
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Protein Isoforms
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trichostatin A