Abstract
In the present study, the antioxidative properties of tectorigenin, a metabolite formed by transformation of tectoridin by intestinal microflora, were investigated. Tectorigenin was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of tectorigenin protected the viability of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) via activation of extracellular signal regulated kinase (ERK) pathway. Furthermore, tectorigenin reduced the apoptotic cells formation and cell cycle arrest at G2/M phase induced by H2O2. Tectorigenin increased the activities of cellular antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase, and also increased their protein level. Taken together, these findings suggest that tectorigenin protected V79-4 cells against H2O2 damage, by enhancing the antioxidative activity and by activating ERK pathway.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Apoptosis / drug effects
-
Bacteroides / metabolism
-
Biotransformation
-
Blotting, Western
-
Catalase / metabolism
-
Cell Cycle / drug effects
-
Cell Line
-
Cell Survival / drug effects
-
Culture Media, Serum-Free / pharmacology
-
Dose-Response Relationship, Drug
-
Enzyme Activation / drug effects
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Free Radical Scavengers / pharmacology
-
Glutathione Peroxidase / metabolism
-
Humans
-
Hydrogen Peroxide / pharmacology*
-
Intestines / microbiology
-
Isoflavones / metabolism
-
Isoflavones / pharmacology*
-
Lipid Peroxidation / drug effects
-
Oxidants / pharmacology
-
Oxidative Stress / drug effects*
-
Superoxide Dismutase / metabolism
-
Transcription Factor AP-1 / metabolism
Substances
-
Culture Media, Serum-Free
-
Free Radical Scavengers
-
Isoflavones
-
Oxidants
-
Transcription Factor AP-1
-
tectorigenin
-
tectoridin
-
Hydrogen Peroxide
-
Catalase
-
Glutathione Peroxidase
-
Superoxide Dismutase
-
Extracellular Signal-Regulated MAP Kinases