Abstract
An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Computer Systems
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DNA Fingerprinting / instrumentation*
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DNA Fingerprinting / methods
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Equipment Design
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Equipment Failure Analysis
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Feasibility Studies
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Fluorescence Resonance Energy Transfer / instrumentation*
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Fluorescence Resonance Energy Transfer / methods
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In Situ Hybridization, Fluorescence / instrumentation*
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In Situ Hybridization, Fluorescence / methods
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Microfluidic Analytical Techniques / instrumentation*
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Microfluidic Analytical Techniques / methods
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Miniaturization
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Oligonucleotide Array Sequence Analysis / instrumentation*
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Oligonucleotide Array Sequence Analysis / methods
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Reproducibility of Results
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Sensitivity and Specificity
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Lösungen