Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread

J Bacteriol. 2006 Feb;188(4):1620-7. doi: 10.1128/JB.188.4.1620-1627.2006.

Abstract

The role in virulence of the Shigella flexneri ospB-phoN2 operon has been evaluated. Here we confirm that OspB is an effector and show that apyrase, the product of phoN2, may be a virulence factor, since it is required for efficient intercellular spreading. Apyrase may be important in a deoxynucleoside triphosphate-hydrolyzing activity-independent manner, suggesting that it may act as an interaction partner in the process of IcsA localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apyrase / genetics
  • Apyrase / physiology*
  • Bacterial Outer Membrane Proteins / physiology
  • Bacterial Proteins / metabolism*
  • Biological Transport
  • DNA-Binding Proteins / metabolism*
  • Operon
  • Shigella flexneri / metabolism*
  • Shigella flexneri / pathogenicity
  • Transcription Factors / metabolism*
  • Virulence Factors

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA-Binding Proteins
  • Transcription Factors
  • Virulence Factors
  • virG protein, Shigella flexneri
  • Apyrase