Abstract
In almost all physiological and pathological situations, cells migrate through three-dimensional environments, yet most studies of cell motility have used two-dimensional substrates. It is clear that two-dimensional substrates do not mimic the in vivo environment accurately, and recent work using three-dimensional environments has revealed many different mechanisms of cell migration (Abbott, 2003; Sahai and Marshall, 2003; Wolf et al., 2003). This chapter will describe methods for generating three-dimensional matrices suitable for studying cell motility, methods for imaging the morphology of motile cells in situ, and methods for quantifying cell migration through three-dimensional environments.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amides / pharmacology
-
Animals
-
Carcinoma, Squamous Cell
-
Cell Culture Techniques
-
Cell Line
-
Cell Line, Tumor
-
Cell Movement / drug effects
-
Cell Movement / physiology*
-
Collagen / metabolism
-
Collagen / ultrastructure
-
Collagen Type I / ultrastructure
-
Drug Combinations
-
Extracellular Matrix / physiology*
-
Humans
-
Intracellular Signaling Peptides and Proteins
-
Laminin / metabolism
-
Laminin / ultrastructure
-
Melanoma
-
Microscopy, Confocal / methods
-
Microscopy, Electron, Scanning
-
Protein Serine-Threonine Kinases / antagonists & inhibitors
-
Proteoglycans / metabolism
-
Proteoglycans / ultrastructure
-
Pyridines / pharmacology
-
rho-Associated Kinases
Substances
-
Amides
-
Collagen Type I
-
Drug Combinations
-
Intracellular Signaling Peptides and Proteins
-
Laminin
-
Proteoglycans
-
Pyridines
-
matrigel
-
Y 27632
-
Collagen
-
Protein Serine-Threonine Kinases
-
rho-Associated Kinases