Crystal structure of non-fused glutathione S-transferase from Schistosoma japonicum in complex with glutathione

Protein Pept Lett. 2005 Oct;12(7):709-12. doi: 10.2174/0929866054696154.

Abstract

Schistosoma japonicum glutathione S-transferase (SjGST) is a common fusion tag in recombinant protein production, and its 3-dimensional structure has been studied in the context of drug design. We have determined the crystal structure of non-fused SjGST complexed with glutathione, and compare it to complexes between glutathione and SjGST fusion proteins.

MeSH terms

  • Animals
  • Crystallography, X-Ray
  • Glutathione / chemistry*
  • Glutathione / metabolism*
  • Glutathione Transferase / chemistry*
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism*
  • Models, Molecular
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Schistosoma japonicum / enzymology*
  • Schistosoma japonicum / genetics

Substances

  • Recombinant Proteins
  • Glutathione Transferase
  • Glutathione