Diapause-associated metabolic traits reiterated in long-lived daf-2 mutants in the nematode Caenorhabditis elegans

Mech Ageing Dev. 2006 May;127(5):458-72. doi: 10.1016/j.mad.2006.01.006. Epub 2006 Mar 7.

Abstract

The longevity of the Caenorhabditis elegans diapausal dauer larva greatly exceeds that of the adult. Dauer formation and adult ageing are both regulated by insulin/IGF-1 signaling (IIS). Reduced IIS, e.g. by mutation of the daf-2 insulin/IGF-1 receptor gene, increases adult lifespan. This may reflect mis-expression in the adult of dauer longevity-assurance processes. Since IIS plays a central role in the regulation of metabolism, metabolic alterations shared by dauer larvae and daf-2 adults represent candidate mechanisms for lifespan determination. We have conducted a detailed comparison of transcript profile data from dauers and daf-2 mutant adults, focusing on expression of metabolic pathway genes. Our results imply up-regulation in both dauers and daf-2 mutant adults of gluconeogenesis, glyoxylate pathway activity, and trehalose biosynthesis. Down-regulation of the citric acid cycle and mitochondrial respiratory chain occurs in dauers, but not daf-2 adults. However, the F(1) ATPase inhibitor was up-regulated in both, implying enhanced homeostasis in conditions where mitochondria are stressed. Overall, the data implies increased conversion of fat to carbohydrate, and conservation of ATP stocks in daf-2 mutant adults, suggesting a state of increased energy availability. We postulate that this fuels increased somatic maintenance activity, as suggested by the disposable soma theory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / metabolism
  • Caenorhabditis elegans Proteins / physiology*
  • Citric Acid / metabolism
  • Citric Acid Cycle
  • Electron Transport
  • Forkhead Transcription Factors
  • Gene Expression Regulation*
  • Gluconeogenesis
  • Glycolysis
  • Models, Biological
  • Mutation*
  • Oligonucleotide Array Sequence Analysis
  • Proton-Translocating ATPases / metabolism
  • Receptor, Insulin / metabolism
  • Receptor, Insulin / physiology*
  • Signal Transduction
  • Transcription Factors
  • Trehalose / metabolism
  • Triglycerides / metabolism
  • Up-Regulation

Substances

  • Caenorhabditis elegans Proteins
  • Forkhead Transcription Factors
  • Transcription Factors
  • Triglycerides
  • daf-16 protein, C elegans
  • Citric Acid
  • Trehalose
  • DAF-2 protein, C elegans
  • Receptor, Insulin
  • Proton-Translocating ATPases