Identification of a new allele, HLA-DRB5*0113, through three different molecular biology techniques

E Garino; M Berrino; F Bertinetto; P Caropreso; R Chidichimo; E Dametto; M E Fasano; E Frisaldi; G Mazzola; F Tondat; M Boccadoro; B Bruno; A Amoroso

doi:10.1111/j.1399-0039.2006.00588.x

Identification of a new allele, HLA-DRB5*0113, through three different molecular biology techniques

Tissue Antigens. 2006 May;67(5):427-9. doi: 10.1111/j.1399-0039.2006.00588.x.

Authors

E Garino¹, M Berrino, F Bertinetto, P Caropreso, R Chidichimo, E Dametto, M E Fasano, E Frisaldi, G Mazzola, F Tondat, M Boccadoro, B Bruno, A Amoroso

Affiliation

¹ Transplantation Immunology, San Giovanni Battista Hospital, Turin, Italy. [email protected]

PMID: 16671952
DOI: 10.1111/j.1399-0039.2006.00588.x

Abstract

A new HLA-DRB5 allele, HLA-DRB5*0113, has been identified in an Italian patient during routine HLA typing in order to activate a bone marrow donor search. HLA typing was performed by different molecular biology techniques, and the results showed that the HLA-DRB5*0113 allele differs from HLA-DRB5*010101 allele for three nucleotide substitutions at codons 57 (GAC-->GAT; Asp) and 58 (GCT-->GAG; Ala-->Glu) of exon 2.

MeSH terms

Alanine / chemistry
Alanine / genetics
Alleles
Amino Acid Sequence
Amino Acid Substitution
Base Sequence
Bone Marrow Transplantation / immunology*
Glutamic Acid / chemistry
Glutamic Acid / genetics
HLA-DR Antigens / genetics*
HLA-DRB5 Chains
Histocompatibility Testing / methods*
Humans
Male
Middle Aged
Molecular Sequence Data
Mutation

Substances

HLA-DR Antigens
HLA-DRB5 Chains
Glutamic Acid
Alanine

Associated data

GENBANK/AY457037