Abstract
Studies suggest an association between consumption of a high-cholesterol diet and periodontitis. We addressed the mechanism by which high dietary cholesterol could be detrimental to periodontal health in a rat model. Feeding a high-cholesterol diet augmented the effects of bacterial pathogens and their products (e.g., lipopolysaccharide and proteases) on production of pro-inflammatory cytokines in fibroblasts. High dietary cholesterol also increased mitochondrial 8-hydroxydeoxyguanosine in the periodontal tissues. These results suggest that excessive tissue oxidative damage induced by high dietary cholesterol could potentiate pro-inflammatory cytokine production by fibroblasts stimulated with bacterial pathogens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Hydroxy-2'-Deoxyguanosine
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Animal Feed
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Animals
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Cholesterol / administration & dosage*
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Cholesterol / blood
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Cholesterol / pharmacology*
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / metabolism
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Disease Models, Animal
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Fibroblasts
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Interleukin-1 / metabolism
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Male
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Mitochondria / metabolism
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Oxidation-Reduction / drug effects
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Periodontitis / chemically induced
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Periodontitis / metabolism*
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Periodontitis / microbiology
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Periodontitis / pathology*
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Rats
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Rats, Wistar
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Triglycerides / blood
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Interleukin-1
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Triglycerides
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Tumor Necrosis Factor-alpha
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8-Hydroxy-2'-Deoxyguanosine
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Cholesterol
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Deoxyguanosine