Autoradiographic visualisation of [3H]DTG binding to sigma receptors, [3H]TCP binding sites, and L-[3H]glutamate binding to NMDA receptors in human cerebellum

Neurosci Lett. 1991 Apr 29;125(2):143-6. doi: 10.1016/0304-3940(91)90012-i.

Abstract

The autoradiographic distributions of [3H]1,3-di-ortho-tolyguanidine ([3H]DTG), [3H]1-[1-(2-thienyl) cyclohexyl] piperidine ([3H]TCP) and L-[3H]glutamate were studied in the human cerebellum. [3H]DTG is a selective label for the sigma receptor, while L-[3H]glutamate binding was carried out under conditions selective for the N-methyl-D-aspartate (NMDA) receptor. [3H]TCP binding sites and sigma receptors showed marked enrichment in the Purkinje cell layer, while L-[3H]glutamate-labelled NMDA receptors showed virtually no binding in the Purkinje cell layer. The results confirm the existence of [3H]TCP binding sites which are not linked to NMDA receptors in the human cerebellum, having a distribution which is more similar to that of the haloperidol-sensitive sigma receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography / methods
  • Female
  • Glutamates / metabolism*
  • Glutamic Acid
  • Guanidines / metabolism*
  • Humans
  • Male
  • Middle Aged
  • Phencyclidine / analogs & derivatives*
  • Phencyclidine / metabolism
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Receptors, Neurotransmitter / metabolism*
  • Receptors, Opioid / metabolism*
  • Receptors, Phencyclidine
  • Receptors, sigma
  • Tritium

Substances

  • Glutamates
  • Guanidines
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Neurotransmitter
  • Receptors, Opioid
  • Receptors, Phencyclidine
  • Receptors, sigma
  • Tritium
  • Glutamic Acid
  • tenocyclidine
  • Phencyclidine
  • 1,3-ditolylguanidine