Blood T-cell repertoire in idiopathic nephrotic syndrome recurrence following kidney transplantation

Am J Transplant. 2006 Sep;6(9):2144-51. doi: 10.1111/j.1600-6143.2006.01415.x.

Abstract

Corticosteroid resistant idiopathic nephrotic syndrome (CR-INS) is a glomerulopathy that recurs after kidney transplantation in 30-50% of patients, suggesting the involvement of systemic albuminuric factors, probably produced by activated T cells. We investigated peripheral T-cell selection and expansion before and after transplantation to identify and characterize T-lymphocyte patterns potentially associated with INS recurrence. We used a combined qualitative and quantitative assessment of Vbeta mRNA alterations at the level of the complementary determining region 3-length distribution (CDR3-LD) of the T-cell receptor (TCR). Peripheral blood mononuclear cells (PBMC) were collected from 18 CR-INS patients (8 with recurrence and 10 without recurrence) on the day of transplantation as well as at 1 month, 1 year and 5 years after transplantation, and Vbeta transcriptomes were analyzed. Our data show that blood T cells from patients with INS recurrence display a TCR repertoire that is stable in time and has a similar level of CDR3-LD alterations as the T-cell repertoire of control patients, both before and after transplantation. These results suggest that the process of INS recurrence does not involve TCR activation or specific clonal expansion of T cells. However, these results do not exclude a role for T cells in the production of an albuminuric factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Female
  • Humans
  • Kidney Transplantation / adverse effects*
  • Kidney Transplantation / immunology
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Nephrotic Syndrome / etiology*
  • Nephrotic Syndrome / immunology
  • Nephrotic Syndrome / pathology
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Antigen, T-Cell / immunology*
  • Recurrence
  • T-Lymphocyte Subsets / immunology*

Substances

  • RNA, Messenger
  • Receptors, Antigen, T-Cell