Abstract
This report describes specific T cell responses to HIV-1 CRF01_AE Env and A Gag peptides in 20 HIV-1 CRF01_AE-infected Thai individuals using an interferon-gamma (IFN-gamma) enzyme-linked immunospot (ELISpot) assay. Twenty-six potentially novel HLA class I-restricted CD8+ T cell epitopes were identified in 14/20 subjects. Fine mapping analysis using the chromium release cytotoxic T lymphocyte (CTL) assay revealed a novel HLA-Cw0602 restricted epitope of HIV-1 CRF01_AE Env (NAKTIIVHL) and a previously identified HIV-1 A Gag epitope (ATLEEMMTA) with a novel HLA-A0206 restriction.
MeSH terms
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CD8-Positive T-Lymphocytes / virology*
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Epitopes, T-Lymphocyte / immunology
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Epitopes, T-Lymphocyte / isolation & purification*
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Gene Products, env / chemistry
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Gene Products, env / immunology
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Gene Products, gag / chemistry
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Gene Products, gag / immunology
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HIV Infections / blood
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HIV Infections / immunology*
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HIV Seropositivity
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HIV-1 / genetics
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HIV-1 / immunology*
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HLA-A Antigens / genetics
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HLA-C Antigens / genetics
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Histocompatibility Antigens Class I / genetics
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Humans
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Interferon-gamma / isolation & purification
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Sequence Analysis, Protein
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Thailand
Substances
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Epitopes, T-Lymphocyte
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Gene Products, env
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Gene Products, gag
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HLA-A Antigens
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HLA-C Antigens
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Histocompatibility Antigens Class I
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Interferon-gamma