PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia

J Clin Microbiol. 2007 Mar;45(3):1035-7. doi: 10.1128/JCM.02144-06. Epub 2007 Jan 17.

Abstract

This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is the first study in Australia using molecular techniques to determine the presence of E. histolytica, E. dispar, and E. moshkovskii.

MeSH terms

  • Adult
  • Animals
  • Australia / epidemiology
  • DNA, Protozoan / analysis
  • DNA, Protozoan / isolation & purification
  • Dysentery, Amebic / epidemiology
  • Dysentery, Amebic / parasitology*
  • Entamoeba / classification
  • Entamoeba / genetics
  • Entamoeba / isolation & purification*
  • Entamoeba histolytica / classification
  • Entamoeba histolytica / genetics
  • Entamoeba histolytica / isolation & purification*
  • Entamoebiasis / epidemiology
  • Entamoebiasis / parasitology*
  • Feces / parasitology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods*

Substances

  • DNA, Protozoan