[A method to produce the animal model of diabetic cardiomyopathy]

Wei Sheng Yan Jiu. 2006 Nov;35(6):707-11.
[Article in Chinese]

Abstract

Objective: To study a simple and scientific method to produce the animal model of diabetic cardiomyopathy.

Methods: Injecting the streptozotocin (STZ) liquid at the left iliac region only once in the model group, observing the general condition, the change of eating level, drinking level, urine level and weight, examining the level of creatine kinase ( CK- MB) , lactate dehydrogenase (LDH-1 ) , insulin, blood sugar and urine sugar. Observe the cardiac muscle ultrastructure, the large vessel tunica intima and electrocardiogram.

Results: After injecting the STZ 3 weeks, the model rats appear morbid, the value of eating, drinking, urine are increased and weight decreased. The model rat blood sugar, urine sugar, CK and LDH levels are higher than control rat. The model rat insulin level is lower than control group. The model rat cardiac muscle ultrastructure accord with the diabetic cardiomyopathy' s, and the fibrous or atheromatous plaque do not been found at the large vessel tunica intima.

Conclusion: The SD model rat appear the diabetic cardiomyopathy condition by injected the STZ (55mg/kg) at the left iliac region only once in 3 weeks. It is a simple and scientific method to produce the animal model of diabetic cardiomyopathy.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Cardiomyopathies / etiology*
  • Cardiomyopathies / physiopathology
  • Diabetes Mellitus, Experimental / complications*
  • Female
  • Injections, Intraperitoneal
  • Male
  • Myocardium / enzymology
  • Rats
  • Rats, Sprague-Dawley
  • Streptozocin / administration & dosage

Substances

  • Blood Glucose
  • Streptozocin