Purification, crystallization and preliminary crystallographic analysis of a GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):239-41. doi: 10.1107/S1744309107008500. Epub 2007 Feb 28.

Abstract

A predicted GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus, termed SsGBP, has been cloned and overexpressed in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion technique in the presence of 0.05 M cadmium sulfate and 0.8 M sodium acetate pH 7.5. A single-wavelength anomalous dispersion data set was collected to a maximum resolution of 2.0 A using a single cadmium-incorporated crystal. The crystal form belongs to space group P2(1)2(1)2(1), with approximate unit-cell parameters a = 65.0, b = 72.6, c = 95.9 A and with a monomer in the asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaeal Proteins / analysis
  • Archaeal Proteins / chemistry*
  • Archaeal Proteins / isolation & purification
  • Crystallization
  • Crystallography, X-Ray / methods*
  • GTP-Binding Proteins / analysis
  • GTP-Binding Proteins / chemistry*
  • GTP-Binding Proteins / isolation & purification
  • Hot Temperature*
  • Sulfolobus solfataricus / chemistry*
  • Sulfolobus solfataricus / growth & development

Substances

  • Archaeal Proteins
  • GTP-Binding Proteins