Differential requirement of ATP and extra-ribosomal proteins for ribosome inactivation by eight RNA N-glycosidases

Biochem Biophys Res Commun. 1992 Jan 31;182(2):579-82. doi: 10.1016/0006-291x(92)91771-h.

Abstract

The requirement of ATP and extra-ribosomal proteins for the inactivation of ribosomes by eight plant RNA N-glycosidases [ribosome-inactivating proteins (RIPs)] was investigated. Tritin, pokeweed antiviral protein and barley RIP depend, as gelonin [Sperti, S., Brigotti, M., Zamboni, M., Carnicelli, D. and Montanaro, L. (1991) Biochem. J., 277, 281-284], on the presence of ATP and extra-ribosomal proteins for full inactivation of ribosomes, while bryodin, lychnin, momordin, momorcochin and saporin inactivate isolated Artemia salina ribosomes suspended in buffer saline.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Glycoside Hydrolases / pharmacology*
  • Kinetics
  • Plant Proteins / pharmacology*
  • Plants / enzymology*
  • Poly U / metabolism
  • Protein Biosynthesis / drug effects*
  • RNA, Ribosomal, 28S / metabolism*
  • Rabbits
  • Reticulocytes / drug effects
  • Reticulocytes / metabolism
  • Ribosomes / drug effects*
  • Ribosomes / metabolism

Substances

  • Plant Proteins
  • RNA, Ribosomal, 28S
  • Poly U
  • Adenosine Triphosphate
  • Glycoside Hydrolases